Development of a method for direct quantification of cytomegalovirus antigenemia by flow cytometry.
نویسندگان
چکیده
Cytomegalovirus (CMV) antigenemia was directly detected in polymorphonuclear leukocytes (PMNLs) from transplant recipients by using flow cytometry (FC). Two fixation and permeabilization methods and seven anti-CMV monoclonal antibodies (MAbs) were evaluated. 1C3, SL20, and NEA-9221 MAbs were more efficacious. The antigenemia detection threshold of FC was 0.05% positive PMNLs, and percentages correlated well with DNA viral load and the appearance of clinical symptoms.
منابع مشابه
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عنوان ژورنال:
- Journal of clinical microbiology
دوره 35 10 شماره
صفحات -
تاریخ انتشار 1997